Technical Data
A3550-07A
Arginine, symmetric, dimethyl (SYM11)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 1-2ug/ml of this lot detected numerous proteins including SmD3 in RIPA lysates from Jurkat cells.
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes SmD3 at ~16kD, and other proteins that contain arginines that are symmetrically dimethylated. Specificity of symmetrically dimethylated arginine was determined by ELISA with unmodified and symmetrically dimethylarginine containing peptides. Species Crossreactivity: Human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Boisvert, F.M., et al., J. Cell. Biol. 159: 957-969, 2002.