Technical Data
ARHGAP1 (Rho GTPase-activating Protein 1, Rho-type GTPase-activating Protein 1, MacGAP)
ARHGAP1(also p50-RhoGAP and CDC42 GTPase-activating protein) is a 50kD member of the ARHGAP family of proteins. It is preferentially expressed in epithelium, and serves as a cytoplasmic protein that deactivates Rho-family GTPases. It does so by contributing an Arg residue (Arg282)to Cdc42 and RhoA, which activates their intrinsic GTPase activity and converts GTP-associated molecules into inactivating GDP molecules. Human ARHGAP1 is 439aa in length and contains one CRAL-TRIO domain that binds small lipophilic molecules (aa53-218), an SH3 binding site (aa228-238) and one C-terminal RhoGAP domain (aa244-431). There are phosphorylation sites at Ser51 and Tyr65. There is one potential splice form with a 24aa substitution for (aa1-150)coupled to a 26aa substitution for (aa300-439), Over (aa1-62, human ARHGAP1 shares 96% aa identity with mouse ARHGAP1.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
Not determined
Recombinant corresponding to aa1-62 from human ARHGAP1 expressed in E. coli (Q07960).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Recognizes human, mouse and rat ARHGAP1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.