		Technical Data
A3576-98E	Arrestin 1, beta (ARRB1, Beta-arrestin-1, ARR1, Arrestin beta-1)

Description:
Beta-arrestin 1 is a 418aa containing signaling neurotransmitter that belongs to the arrestin family with an arrestin N-terminal and an arrestin C-terminal domain. A regulator of beta-adrenergic receptor function, Beta-arrestin 1 seems to bind to phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins. It is also reported to act as an activator of the lymphoid enhancer factor (LEF) transcription factor. Beta-arrestin 1 plays a crucial role in ubiquitination and down-regulation of the insulin-like growth factor-1 receptor by acting as an adaptor for the MDM2 E3 ligase. Purified beta-arrestin inhibits the signaling function of BARK-phosphorylated beta-adrenergic receptors by more than 75%, but not that of rhodopsin, and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most tissues.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Testis lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa320-370 of human Beta-arrestin 1.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes human Beta-arrestin 1. Species Crossreactivity: bovine, human, mouse, rat, zebrafish. Species sequence homology: chicken, xenopus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lohse, MJ. et al. Science. 248:1547-1550 (1990). 2. Buchanan, FG. et al. Proc. Nat. Acad. Sci. 103:1492-1497 (2006). 3. Kovacs, JJ. et al. Science. 320:1777-1781 (2008).