Technical Data
A3576-98E
Arrestin 1, beta (ARRB1, Beta-arrestin-1, ARR1, Arrestin beta-1)
Description:
Beta-arrestin 1 is a 418aa containing signaling neurotransmitter that belongs to the arrestin family with an arrestin N-terminal and an arrestin C-terminal domain. A regulator of beta-adrenergic receptor function, Beta-arrestin 1 seems to bind to phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins. It is also reported to act as an activator of the lymphoid enhancer factor (LEF) transcription factor. Beta-arrestin 1 plays a crucial role in ubiquitination and down-regulation of the insulin-like growth factor-1 receptor by acting as an adaptor for the MDM2 E3 ligase. Purified beta-arrestin inhibits the signaling function of BARK-phosphorylated beta-adrenergic receptors by more than 75%, but not that of rhodopsin, and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most tissues.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Testis lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa320-370 of human Beta-arrestin 1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Beta-arrestin 1. Species Crossreactivity: bovine, human, mouse, rat, zebrafish. Species sequence homology: chicken, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lohse, MJ. et al. Science. 248:1547-1550 (1990). 2. Buchanan, FG. et al. Proc. Nat. Acad. Sci. 103:1492-1497 (2006). 3. Kovacs, JJ. et al. Science. 320:1777-1781 (2008).