Technical Data
Arrestin 2, beta (ARRB2, ARB2, ARR2, DKFZp686L0365, HGNC:712)
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms: beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000 Bands of 49kD and 47kD bands are observed representing beta-arrestin and beta-arrestin 2, respectively.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
PabIgGAffinity Purified
100ug-20°CBlue IceRabbit
Synthetic peptide corresponding to amino acid residues 384-397, CD(384)DIVFEDFARLRLK(397) from human beta-arrestin 2.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 1 mg/ml BSA, 0.05% sodium azide.
Detects recombinant rat beta-arrestin and beta-arrestin 2. Does not detect visual or cone arrestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mundell SJ et al. Characterization of arrestin expression and function. Methods Enzymol 343:600-11 (2002). 2. McDonald PH & Lefkowitz RJ Beta-Arrestins: new roles in regulating heptahelical receptors' functions. Cell Signal 13:683-9 (2001). 3. Penn RB et al. Arrestin specificity for G protein-coupled receptors in human airway smooth muscle. J Biol Chem 276:32648-56 (2001).