Technical Data
ARRB2 (Beta-arrestin-2, Arrestin beta-2, ARB2, ARR2)
Beta-arrestin-2 or ARRB2 is a 409aa adaptor protein belonging to the arrestin family with an N- arrestin domain and a C-arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta-adrenergic receptor A function, thus enhancing beta2AR receptor-mediated nuclear translocation of ERK. It binds to phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. ARRB2 is widely expressed in most tissues, particularly neuronal tissues and spleen.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa281-298 DNREKRGLALDGKLKHED of human ARRB2.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human ARRB2. Species Crossreactivity: chimpanzee, canine, porcine, rhesus monkey. Species sequence homology: chicken, bovine, equine, macaque, mouse, pufferfish, rabbit, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Attramadal, H. et al. J. Biol. Chem. 267:17882-17890 (1992). 2. Alloway, PG. et al. Neuron. 28:129-138 (2000). 3. Chen, W. et al. Science. 301:1394-1397 (2003). 4. Chen, W. et al. Science. 306:2257-2260 (2004).