Technical Data
A3577-02D
ARRB2 (Beta-arrestin-2, Arrestin beta-2, ARB2, ARR2)
Description:
Beta-arrestin-2 or ARRB2 is a 409aa adaptor protein belonging to the arrestin family with an N- arrestin domain and a C-arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta-adrenergic receptor A function, thus enhancing beta2AR receptor-mediated nuclear translocation of ERK. It binds to phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. ARRB2 is widely expressed in most tissues, particularly neuronal tissues and spleen.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa281-298 DNREKRGLALDGKLKHED of human ARRB2.
Purity:
Serum.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human ARRB2. Species Crossreactivity: chimpanzee, canine, porcine, rhesus monkey. Species sequence homology: chicken, bovine, equine, macaque, mouse, pufferfish, rabbit, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Attramadal, H. et al. J. Biol. Chem. 267:17882-17890 (1992). 2. Alloway, PG. et al. Neuron. 28:129-138 (2000). 3. Chen, W. et al. Science. 301:1394-1397 (2003). 4. Chen, W. et al. Science. 306:2257-2260 (2004).