		Technical Data
A3780	ASCT1 A (MYS, Amino Acid Transporter)

Description:
ASCT1 is also known as MYS. It is involved in glutamine transport. ASCT1 overexpression is associated with better survival in non-small cell stage I lung carcinomas. It is associated with worse survival in carcinomas of the esophagus.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (FFPE): 5ug/ml; After deparaffinization and rehydration, tissue sections should be treated with an antigen retrieval method, such as steaming in 10mM citrate buffer pH 6.0 for 30 minutes. Block nonspecific binding with PBS, 2% normal goat serum and 1% BSA. Incubate sections at RT for 2 hours with 5ug/ml of A3780 diluted in PBS, pH 7.4, 0.1% BSA.
Immunocytochemistry: HepG2 cells
Western Blot: 5ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~2mg/ml

Immunogen:

Synthetic peptide corresponding to 12aa sequence of the N-terminus of human neutral amino acid transporter.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.4, 0.075% BSA, 0.025% sodium azide.

Specificity:

Recognizes human ASCT1. Strong ASCT1 immunostaining is detectable in many human cancers including those of the colon, lung, esophagus and breast. Weak, and rarely strong, positive ASCT1 immunostaining in many normal human cell types.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Arriza, et al., J. Biol. Chem. 268(21): 15,329-15,332 (1993). 2. Bode, et al., Hepatology 21: 511-520 (1995). 3. Wasa, et al., Ann. Surg. 224: 189-197 (1996). 4. Shafqat, S., et al., J. Biol. Chem. 268: 15,351-15,355 (1993).