Technical Data
A3883-01C
Aspergillus
Description:
A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. A3883-01C has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. A3880-01C has also been used for the identification of aspergillosis in human tissue sections.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:300
Requires protein digestion pretreatment of paraffin sections.
Western Blot: Detects a band of ~106kD of Aspergillus fumigatus wall fraction (WF).
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c ABom mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM10B218Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Wall fraction (WF) of Aspergillus fumigatus.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes members of the Aspergillus spp including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae. Does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis. J. Vet. Diagn. Invest. 8: 68-75. 2. Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests. APMIS. 104: 241-258. 3. Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies. J. Pathol. 181: 100-105.