Technical Data
A3883-45
Asporin (ASPN, Asporin (LRR class 1), FLJ20129, Periodontal Ligament Associated Protein 1, PLAP1, Small Leucine-rich Protein 1C, SLRR1C)
Description:
ASPN, or Asporin, belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin. Asporin is expressed in a variety of human tissues with higher levels in osteoarthritic articular cartilage, aorta, uterus, heart and liver. The function of Asporin is unclear, but available data indicates a collagen binding capacity.

Applications:
Suitable for use in ELISA, Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000.
Immunocytochemistry/Immunofluorescence: 1-3ug/ml
Western Blot: 1-3ug/ml. ~ 40kD band observed in human bone marrow lysates (calculated MW of 43.4kD according to NP_060150.3).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide C-IHENKVKKIQKDT from the the Internal region of human asporin (Accession # NP_060150).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Tested: Human. Expected from sequence similarity: Human, Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kizawa H, Kou I, Iida A, Sudo A, Miyamoto Y, Fukuda A, Mabuchi A, Kotani A, Kawakami A, Yamamoto S, Uchida A, Nakamura K, Notoya K, Nakamura Y, Ikegawa S.
An aspartic acid repeat polymorphism in asporin inhibits chondrogenesis and increases susceptibility to osteoarthritis.
Nat Genet. 2005 Feb;37(2):138-144. Epub 2005 Jan 09.
PMID: 15640800