Technical Data
A3883-45
Asporin (ASPN, Asporin (LRR class 1), FLJ20129, Periodontal Ligament Associated Protein 1, PLAP1, Small Leucine-rich Protein 1C, SLRR1C)
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Technical Data |
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A3883-45 |
Asporin (ASPN, Asporin (LRR class 1), FLJ20129, Periodontal Ligament Associated Protein 1, PLAP1, Small Leucine-rich Protein 1C, SLRR1C) |
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Description: ASPN, or Asporin, belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin. Asporin is expressed in a variety of human tissues with higher levels in osteoarthritic articular cartilage, aorta, uterus, heart and liver. The function of Asporin is unclear, but available data indicates a collagen binding capacity. Applications: Suitable for use in ELISA, Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000. Immunocytochemistry/Immunofluorescence: 1-3ug/ml Western Blot: 1-3ug/ml. ~ 40kD band observed in human bone marrow lysates (calculated MW of 43.4kD according to NP_060150.3). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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