Technical Data
AT Rich Interactive Domain 1A (ARID1A, B120, BAF250, BAF250a, BM029, C1orf4, P270, SWI-like, SMARCF1)
This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
The epitope recognized by this antibody maps to a region between residue 675 and 725 of human AT rich interactive domain 1A (BRG1-associated factor 250kD) using the numbering given in entry NP_006006.3 (GeneID 8289).
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
Species Crossreactivity: Human. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.