Technical Data
A3913-35
ARID5B (MRF2, AT-rich Interactive Domain-containing Protein 5B, ARID Domain-containing Protein 5B, MRF1-like Protein, MRF-2, Modulator Recognition Factor 2, DESRT)
Description:
ARID5B belongs to the ARID (A-T rich interaction domain) family of transcription factors. The ARID (A-T Rich Interaction Domain) is a helix-turn-helix motif-based DNA-binding domain, which is conserved in all eukaryotes and diagnostic of a family that includes fifteen distinct human proteins. It shows important roles in development, tissue-specific gene expression and proliferation control. This protein has a nonredundant function during growth and in the development of the reproductive system. ARID5B is essential for accumulation of lipid stores in postnatal life. Northern blot analysis shows expression in mouse lung, heart, small intestine, kidney, muscle, and brain.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 2-4ug/ml
Immunohistochemistry (Paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human tonsil, mouse placenta, U87 (human) or NIH 3T3 (mouse) cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a portion of aa500-550 from human ARID5B (Q14865). Species Sequence Homology: mouse, equine; 93%, rat; 87%
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human ARID5B. Species Crossreactivity: chimpanzee, New World monkey and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Whitson, RH. et al Biochem Biophys Res Commun. 312:997-1004 (2003). 2. Patsialou, A. et al. Nucleic Acids Res. 33:66-80 (2005).