Technical Data
ATG3 (Ubiquitin-like-conjugating Enzyme ATG3, Autophagy-related Protein 3, APG3-like, hApg3, Protein PC3-96, APG3, APG3L)
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuoles and has a significant relationship to several neurodegenerative disorders and myopathies in mammals. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. One of APG gene products essential for autophagy in yeast, Apg3p, is a protein-conjugating enzyme for Apg8p lipidation. Its human homologue, APG3L, is identified as an authentic E2 enzyme essential for human Apg8p homologues (i.e. GATE-16, GABARAP, and MAP-LC3). APG3L forms an E1-E2 complex with APG7L and also interacts with APG12L. Human APG3L gene is localized on chromosomal region 3q13.2.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa192-210 (DAILQTRTYDLYITYDKYY) of human ATG3.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human ATG3. Species Crossreactivity: bovine, mouse, rat, xenopus, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Isei, T. et al. J. Biol. Chem. 277:13739-13744 (2002). 2. Gozuacik, D. et al. Oncogene. 23:2891-2906 (2004). 3. Kisen, GO. et al. Carcinogenesis. 14:2501-2505 (1993).