Technical Data
A3965
Atlastin-3 (ATL3)
Description:
Atlastin is a recently characterized, integral protein GTPase similar to members of the dynamin family of large GTPases. It is primarily localized on the Golgi apparatus and regularly considered to contain two transmembrane domains which result in the exposure of both the N-terminal and C-terminal ends to the cytoplasm. Studies have shown atlastin to bind with spastin in biological pathways, such as microtubule-severing leading to axon outgrowth. Mutations in atlastin expression are causal to childhood onset HSP, a group of conditions in which spastic parapalegia occurs. Atlastin has been found in several tissues throughout the body. The most significant levels of atlastin have been observed in the brain and spinal cord.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C/-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Linear peptide corresponding to mouse Atlastin-3 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Atlastin-3. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.