Technical Data
A4000-52
ATPase alpha 1, Na, K
Description:
Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.05-0.1ug/ml detected the a-1 subunit in 20ug of rat brain microsomal preparations using chemiluminescence. To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Immunohistochemistry: 1-10ug/ml on ice-cold methanol-fixed tissue.
Immunoprecipitation: 2ug immunoprecipitated the a-1 subunit from rat brain microsomal preparations.
Optimal dilutions to be determined by the researcher.

Source:
Culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k0.T.1Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRabbitMouse
Concentration:
~1mg/ml
Immunogen:
Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Specificity:
Recognizes rabbit Na+/K+ ATPase alpha-1 subunit. MW: 112kD. Species Crossreactivity: human, monkey, rat, mouse, sheep, canine, porcine and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Capasso, J.M., et al., Proc. Natl. Acad. Sci. USA. 100: 6428-6433 (2003). 2. Mitchell, R., et al., J. Biol. Chem. 278: 33,818-33,830 (2003). 3. Ramsauer, V.P., et al., J. Biol. Chem. 278: 30,142-30,147 (2003). 4. Thevananther, S., et al., J. Biol. Chem. 273: 23,952-23,958 (1998). 5. Thompson, C.B., et al., J. Biol. Chem. 271: 32,653-32,658 (1996).