Technical Data
ATP6V0A3 (V-type Proton ATPase 116kD Subunit a Isoform 3, V-ATPase 116kD Isoform a3, Osteoclastic Proton Pump 116kD Subunit, OC-116kD, OC116, T-cell Immune Regulator 1, T-cell Immune Response cDNA7 Protein, TIRC7, Vacuolar Proton Translocating ATPase 116k
TIRC7 is an 830aa containing novel T cell membrane protein directly involved in T cell activation. A member of the V-ATPase 116kD subunit family and a part of the proton channel of V-ATPases, TIRC7 is a heteromultimeric regulatory enzyme mediating the acidification of the bone:osteoclast interface. Two isoforms of TIRC7 are present which express in osteoclastomas and thymus. Besides immune response and bone resorption, TIRC7 may also act as an upstream regulatory molecule of CTLA4 expression. Defects in TIRC7 are the cause of osteopetrosis autosomal recessive type 1 (OPTB1).

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa5-22 of human TCIRG1 ISYWGEQIGQKIRKITDC.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human ATP6V0A3. Species Crossreactivity: chimpanzee, monkey, mouse. Species sequence homology: chicken, bovine, canine, rat, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Borthwick, KJ. et al. J. Med. Genet. 40:115-121 (2003). 2. Frattini, A. et al. Nature Genet. 25:343-346 (2000). 3. Heinemann, T. et al. Genomics. 57:398-406 (1999).