Technical Data
A4000-94A
ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)
Description:
The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The sarco/endoplasmic reticulum Ca2+ ATPases (SERCAs) mediate Ca2+ uptake into intracellular stores. SERCA mediated Ca2+ uptake induces and maintains muscular relaxation. SERCA1 is expressed in fast-twitch muscles, whereas SERCA2 is expressed in cardiac and slow-twitch muscles. SERCA2 plays an important part in regulating cardiac contractile function.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HEK293 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10H15Purified
SizeStorageShippingSourceHost
100ug4°C/-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified canine cardiac sarcoplasmic reticulum.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human SERCA2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.