Technical Data
A4000-94A
ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)
Description:
The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The sarco/endoplasmic reticulum Ca2+ ATPases (SERCAs) mediate Ca2+ uptake into intracellular stores. SERCA mediated Ca2+ uptake induces and maintains muscular relaxation. SERCA1 is expressed in fast-twitch muscles, whereas SERCA2 is expressed in cardiac and slow-twitch muscles. SERCA2 plays an important part in regulating cardiac contractile function.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml detected SERCA2 in 10ug of HEK293 cell lysates
Optimal dilutions to be determined by the researcher.

Positive Control:
HEK293 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10H15Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purified canine cardiac sarcoplasmic reticulum.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Eizema, k, et al. (2007) J. Histochem. and Cytochem 55(3): 247-254. 2. Zhang, Q., et al. (2005) J. Cell Sci. 117(Pt 4): 673-687. 3. Mearow, K.M., et al. (2993). Mol. Cell Biochem. 121(2): 155-165.