Technical Data
A4005
ATP Synthase, beta
Description:
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATPase (soluble) and the F0 ATPase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta and epsilon subunits. While the F0 consists of a, b and c subunits.

Applications:
Suitable for use in Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1ug/ml for one hour at RT using 10e6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H385Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat brain mitochondria.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the beta chain of rat ATP Synthase. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.