Technical Data
A4189-05
Aurora A Kinase (Aurora-Related Kinase 1, ARK1, Breast Tumor-Amplified Kinase, BTAK, Serine Threonine Kinase 6/15, STK6, STK15)
Description:
A4189-05 reacts with mammalian aurora-A kinase which is a member of the kinase family that is named after the Drosophila aurora kinase Nigg. There are three different kinases (Aurora A, Aurora B and Aurora C) which are involved in centrosome separation. Aurora kinases share a conserved C-terminal kinase catalytic domain but have a different N-terminal non catalytic domain. They show different localization during mitosis and fulfill different functions. The most important feature of these kinases is that they are overexpressed in various primary tumors. For instance Aurora A is significantly downregulated in a subset of breast tumors. Aurora A Kinase is also known as Aurora-related Kinase 1 (ARK1), Breast-tumor-amplified Kinase (BTAK) and Serine/threonine Kinase 6 or 15 (STK6 or STK15).

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10
Western Blot: 1:10
Immunohistochemistry (Frozen and Paraffin): 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b6A482Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant, full length His-tagged Aurora A Kinase (Human).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Aurora A Kinase. Binds to duplicated centrosomes and spindle poles. Does not inhibit the Aurora A Kinase activity. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cremet, J., et al., Mol. Cell Biochem. 243: 123 (2003). 2. Conte, N., et al., Oncogene 22: 8102 (2003).