Technical Data
A4445-11L
Aven (Apoptosis Caspase Activation Inhibitor, Cell Death Regulator Aven, PDCD12)
Description:
PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer
Applications:
Suitable for use in ELISA and Western Blot Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-3000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCD12 protein.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k9C85Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human PDCD12 (254-362aa) purified from E. coli
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human PDCD12 (Programmed cell death 12, AVEN).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Figueroa B Jr, et al: (2004) Biotechnol Bioeng. 85(6), 589-600 2. Cheng EH, et al: (2000) Mol Cell. 6(1), 31-40