Technical Data
B0000-01
B220 (B cell specific isoform of CD45)
Description:
B220 (CD45R) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
B220 (CD45R) is required for t-cell activation through the antigen receptor. The first ptpase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.

Applications:
Suitable for use in Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 10ul/10e6 cells

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM5C12Highly Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Purified mouse LN B cells.
Purity:
Purified by ion exchange chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 500ul sterile 40-50% glycerol, dH2O.
Specificity:
Recognizes mouse B220.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.