Technical Data
B0000-35
B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1)
Description:
As a member of B7 family, B7-H4 (VTCN1) plays a critical role in the regulation of antigen-specific immune responses.B7-H4 is a co-stimulatory protein expressed in activated T cells and is involved in the negative regulation of cell-mediated immunity in peripheral tissues (1). B7-H4 ligation to its receptor BTLA T-lymphocytes results in inhibition of T-cell activation, cytokine secretion, and the development of cytotoxicity (2).B7-H4 protein is typically expressed on the surface of activated T-cells, B-cells, monocytes, and dendritic cells, but not in most normal
tissues.In tissues, B7-H4 is over expressed in breast, endometrial, ovarian, renal cell (RCC), and non-small cell lung carcinomas (NSCLC).B7-H4 has been identified as an important cancer marker for breast and ovarian cancers (3).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Not suitable for Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunoprecipitation: 1:20
Immunohistochemistry (paraffin): 1:250-1:500 antigen retrieval is recommended.
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
Human fetal spleen, thymus and SKBR3 lysates; human tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9H294Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Peptide corresponding to residues near the N-terminal of human B7H4.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, 0.01% sodium azide, 0.05% BSA, 50% glycerol.
Specificity:
Recognizes human B7H4 at ~50kD. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tringler B, et al.,Clinical Cancer Research 11:1842-1848, 2005. 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195(2008). 3. Simon I, et al., Cancer Research 66:1570 1575, 2006.