Technical Data
B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1)
As a member of B7 family, B7-H4 (VTCN1) plays a critical role in the regulation of antigen-specific immune responses.B7-H4 is a co-stimulatory protein expressed in activated T cells and is involved in the negative regulation of cell-mediated immunity in peripheral tissues (1). B7-H4 ligation to its receptor BTLA T-lymphocytes results in inhibition of T-cell activation, cytokine secretion, and the development of cytotoxicity (2).B7-H4 protein is typically expressed on the surface of activated T-cells, B-cells, monocytes, and dendritic cells, but not in most normal
tissues.In tissues, B7-H4 is over expressed in breast, endometrial, ovarian, renal cell (RCC), and non-small cell lung carcinomas (NSCLC).B7-H4 has been identified as an important cancer marker for breast and ovarian cancers (3).

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Not suitable for Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunoprecipitation: 1:20
Immunohistochemistry (paraffin): 1:250-1:500 antigen retrieval is recommended.
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
Human fetal spleen, thymus and SKBR3 lysates; human tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
Peptide corresponding to residues near the N-terminal of human B7H4.
Supplied as a liquid in PBS, 0.01% sodium azide, 0.05% BSA, 50% glycerol.
Recognizes human B7H4 at ~50kD. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tringler B, et al.,Clinical Cancer Research 11:1842-1848, 2005. 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195(2008). 3. Simon I, et al., Cancer Research 66:1570 1575, 2006.