Technical Data
B0003-05C2
Bacillus anthracis PA (Protective Antigen) (Anthrax)
Description:
Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these proteins are toxic by themselves and several studies indicate that the anthrax toxin has the familiar A-B enzymatic binding structure, with PA acting as the binding domain and EF and/or LF acting as the active fragments.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1ug/ml detects 10ng of free peptide
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 16aa near the middle of the Anthrax protective antigen protein (AAF86457).
Purity:
Purified by Immunoaffinity chromatography
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes Bacillus anthracis Protective Antigen, middle region.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schwartz, M.N., (2001), "Recognition and management of anthrax – an update", New Engl. J. Med., 345: 1621-1626. 2. Moayeri, M., et al., (2004), "The roles of anthrax toxin in pathogenesis", Curr. Opin. Microbiol., 7: 19-24. 3. Bradley, K.A., et al., (2001), "Identification of the cellular receptor for anthrax toxin", Nature, 414: 225-229. 4. Scobie, H.M., et al., (2003), "Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor", Proc. Natl. Acad. Sci. USA, 100: 5170-5174. 5. Singh, Y., et al., (1999), "Oligomerization of anthrax toxin protective antigen and binding of lethal factor during endocytotic uptake into mammalian cells", Infect. Immun., 67: 1853-1859.