Technical Data
B0003-40C
Bactericidal Permeability Increasing Proetin (BPI)
Description:
The antimicrobial protein BPI is a protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airways disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21kD bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin.

Applications:
Suitable for use in Flow Cytometry, ELISA and Neutralization. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14E3Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Mouse bacterial permeability increasing protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Specificity:
Recognizes full length human natural and recombinant human Bactericidal Permeability Increasing protein (BPI). Recognizes only free BPI and does not interact with BPI that has formed a complex with LPS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dentener, M et al; Antagonistic effects of lipopolysaccharide binding protein and bactericidal/ permeability increasing protein on lipopolysaccharide-induced cytokine release by mononuclear phagocytes. J Immunol 1993, 151: 4258 2. Dentener, M et al; Characterization of two monoclonal antibodies directed against bactericidal/permeability increasing protein. J Infect Dis 1994, 170: 14833. Dentener, M et al; Bactericidal/permeability-increasing protein, a lipopolysaccharide-specific protein on the surface of human peripheral blood monocytes. J Infect Dis 1996, 173: 252.