Technical Data
Bad Control Proteins
Molecular Biology Storage: -20CShipping: Blue Ice
Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-XL, resulting in cell death (1,2). Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-XL and Bcl-2 (2). Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136 (3,5). Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK (6,7) and mitochondria-anchored PKA (8).

Nonphosphorylated Bad Control Proteins: Nonphosphorylated Bad peptide fusion serves as a negative control. Supplied in SDS Sample Buffer.

Phosphorylated Bad Control Proteins: Phosphorylated Bad peptide fusion serves as a positive control.

Directions for Use:
As controls, we recommend using 10ul of phosphorylated and nonphosphorylated Bad control proteins. Boil for 2 minutes prior to use. 100ul wil be sufficient for 10 blots.
Form: Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25C, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue or phenol red.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) Yang, E. et al. (1995) Cell 80, 285291. (2) Zha, J. et al. (1996) Cell 87, 619628. (3) Datta, S.R. et al. (1997) Cell 91, 231241. (4) Mizushima, S. and Nagata, S. (1990) Nucleic Acids Res. 18, 5322. (5) Peso, L. et al. (1997) Science 278, 687689. (6) Bonni, A. et al. (1999) Science 286, 13581362. (7) Tan, Y. et al. (1999) J. Biol. Chem. 274, 3485934867. (8) Harada, H. et al. (1999) Mol. Cell 3, 413422.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.