Technical Data
B0072-01A
Band 3 Protein
Description:
The Band 3 protein is the most abundant integral protein of human erythrocyte membranes and functions as the major anion transporter polypeptide of erythrocytes. Band 3 is a 90-100 kD protein, its microheterogeneity is ascribed mainly to the structural heterogeneity of the carbohydrate moiety. The C-terminal portion of the molecule spans the membrane several times, and is responsible for anion transport. The N-terminal portion, comprising almost half of the polypeptide chain of Band 3, is located inside the red cell and interacts with cytoskeletal and cytoplasmic proteins such as ankyrin.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Indirect Western Blot (Titer): 1:5000 using human erythrocytes
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a8J283Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Purified, full length native Band 3 protein. Epitope: located in the cytoplasmic pole of the Band 3 molecule, within ~20kD from the N-terminal end. Cellular Localization: Cell Membrane; multi-pass membrane protein.
Purity:
Ascites
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes the cytoplasmic amino-terminal of Band 3 protein (90-100kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20kD. Digestion and aggregation results in detection with multiple molecular weight proteins. Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay. Species Crossreactivity: Human. Does not crossreact with porcine, mouse, bovine, canine, Guinea pig or equine. Does not react with human fibroblast extract (nonerythroid).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Czerwinki M et al. Degradation of the human erythrocyte membrane band 3 studied with monoclonal antibody directed against an epitope on the cytoplasmic fragment of band 3. Eur J Biochem 174:647-54 (1988).