Technical Data
B0132-05
BNC1 (Zinc Finger Protein Basonuclin-1, BNC)
Description:
Bnc 1 is a 994aa transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. It is a zinc finger protein with three separated pairs of zinc fingers and a nuclear localization signal. Bnc 1 is a soluble protein that can shuttle between the nucleus and the cytoplasm, and its location depends on the proliferative potential of the cell. It is expressed relatively uniformly in the nucleoplasm, and phosphorylation on Ser-537 and Ser-541 leads to its cytoplasmic localization. It is present in the basal cell layer of the epidermis, in hair follicles and also in abundance in the germ cells of testis and ovary, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart. Reports suggest that it plays a regulatory role in keratinocyte proliferation and also in rRNA transcription. It is also known to play a role in the differentiation of spermatozoa and oocytes.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 4-7ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human and rat spleen lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa330-380 of human BNC1protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human BNC1. Species Crossreactivity: chimpanzee, monkey. Species sequence homology: bovine, equine, mouse, rat, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vanhoutteghem, A. et al. Proc. Natl. Acad. Sci. USA. 103:12423-12428 (2006). 2. Tseng, H. et al. J. Cell. Sci. 18:3039-3047 (1999). 3. Tseng, H. et al. Proc. Nat. Acad. Sci. 89:10311-10315 (1992).