Technical Data
B0140-01A
Bassoon
Description:
Bassoon is a 420kD protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:400
Immunohistochemistry: Paraffin sections
Immunoprecipitation: 2.5ug
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a8J289Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant fusion protein corresponding to aa738-1035 of rat bassoon expressed as a in E.coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol
Specificity:
Recognizes rat bassoon at ~400kD. Additional bands between 97kD and 400kD haev been identified and are reported to be proteolytic degradation products of bassoon. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tom Dieck, S., Sanmarti-Vila, L., Langnaese, K., Richter, K, Kindler, S., Soyke, A., Wex, H, Smalla, K-H., Kampf, U., Franzer, J-T., Stumm, M., Garner, C.C., Gundelfinger, E.D. (1998) J. Cell Biol. 142: 499-509. 2. Richter, K., Langnaese, K., Kreutz, M.R., Olias, G., Zhai, R., Scheich, H., Garner, C.C., Gundelfinger, E.D. (1999) J. Comp. Neurol. 408: 437-448. 3. Winter, C., tom Dieck, S., Boeckers, T.M., Bockmann, J., Kanmpf, U., Sanmarti-Vila, L., Langnaese, K., Altrock, W., Stumm, M., Soyke, A., Wieacker, P., Garner, C.C., Gundelfinger, E.D. (1999) Genomics 57: 389-397. 4. Cases-Langhoff, C., Voss, B, Garner, A.M., Appeltauer, U., Takei, K., Kindler, S., Veh, R.W., De Camilli, P., Gundelfinger, E.D., Garner, C.C. (1996) Eur. J. Cell Biol 69: 214-223. 5. Phillips, G.R., et al. (2001) Neuron 32: 63-77. 6. Morales, M., Colicos, M.A., Goda, Y. (2000) Neuron 27: 539-550. 7. Ohtsuka, T.; Takao-Rikitsu, E.; Inoue, E.; Inoue, M.; Takeuchi, M.; Matsubara, K.; Deguchi-Tawarada, M., Satoh, K., Morimoto, K., Nakanishi, H., Takai, Y. (2002) J Cell Biol 158(3): 577-590. 8. Shiraishi, Y., Mizutani, A., Mikoshiba, K., Furuichi, T. (2003) Mol Cell Neurosci 22(2): 188-201. 9. Lee, H.K., Takamiya, K., Han, J.S., Man, H., Kim, C.H., Rumbaugh, G., Yu, S., Ding, L., He, C., Petralia, R.S., Wenthold, R.J., Gallagher, M., Huganir, R.L. (2003) Cell 112(5):631-643. 10. Hirbec, H., Francis, J.C., Lauri, S.E., Braithwaite, S.P., Coussen, F., Mulle, C., Dev, K.K., Couthino, V., Meyer, G., Isaac, J.T.R., Collingridge, G.L., Henley, J.M. (2003) Neuron 37(4): 625-638.