Technical Data
BLC, Recombinant, Mouse (B-lymphocyte Chemoattractant, Mouse Equivalent of Human B Cell-Attracting Chemokine-1, BCA-1, CXCL13)
Molecular Biology Storage: -20CShipping: RT
BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes.

Recombinant corresponding to 86aa from mouse BLC expressed in E. coli.

Molecular Weight:

Endotoxin: 0.1ng/ug

Biological Activity:
The biological activity was determined by measuring dose-dependent chemotaxis with murine B lymphocytes. A concentration range of 10-100ng/ml produced significant chemotaxis. BLC, a CXC chemokine, regulates B cell migration to and within lymphoid follicles in most secondary lymphoid organs. The receptor for BLC is CXCR5.

Storage and Stability:
Store at -20C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1-1% BSA. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~98% (SDS-PAGE, HPLC). Purified by sequential chromatography.
Concentration: Not determined
Form: Supplied as a sterile filtered lyophilized powder, carrier free. Reconstitute in sterile, ddH2O, 0.1-1% BSA to 0.5-1mg/mL. Further dilutions should be made in aqueous buffers.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.