Technical Data
BCA-225 (BRST-1)
BCA-225, a 225kD glycoprotein, is secreted by the T47D breast carcinoma cell line. Strong intracytoplasminc staining is seen in primary and metastatic breast carcinoma cell lines, primary and metastatic breast carcinoma tissue and cervical carcinomas.

Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin sections): 1:40 or higher for biotin-based detection systems. Incubate 20 mins. at RT. Tissue pre-treatment not required.
Optimal dilution to be determined by the researcher.

Positive Control:
Human breast carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceHumanMouse
Not determined
Protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
Tissue culture supernatant
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Recognizes human BCA-225.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Imam, S.A. et al., Comparison of two microwave based antigen retrieval solutions in unmasking epitopes in formalin-fixed tissue in immunostaining. Anticancer Res. 15:1153-1158 (1995). 2. Ansai ,S., et al. An immunohistochemical study of BCA-225 in various skin cancers. J Dermatol 21: 20-24 (1994). 3. Ma, C.K., et al. Comparative immunohistochemical study of primary and metastatic carcinomas of the liver. Am J Clin Pathol 99:551-557 (1993).