		Technical Data
B0397-10A	B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)

Description:
BLNK plays a pivotal role in B cell activation through cross-linkage of the B-cell Receptor (BCR), leading to the activation of protein tyrosine kinases (PTKs) including Syk. The resulting tyrosine phosphorylation of BLNK by Syk, provides docking sites for the SH2-containing effector molecules such as Vav, PLC gamma and the adaptor proteins Crb2 and Nck, thereby acting as a central linker protein in many different B cell signalling pathways involved in proliferation, differentiation and apoptosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000, detects two bands of approximately 68 and 70kD in mouse Sp2/0 cell lysates.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
I1903-30U IgG, H&L, X-adsorbed (HRP)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Fusion protein corresponding to amino acids 171-356 of human BLNK.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

Specificity:

Recognizes B-cell Linker Protein (BLNK), a cytosolic adaptor molecule, visualised as two phosphoproteins of 68 and 70kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Fu, C. et al. (1998). BLNK: a central linker protein in B cell activation. Immunity 9(1): 93-103. 2. Xu, S. et al. (2000). B cell development and activation defects resulting in xid-like immunodeficiency in BLNK/SLP-65-deficient mice. Int. Immunol. 12(3): 397-404. 3. Chiu, C.W.et al. (2002). BLNK: molecular scaffolding through ‘cis’-mediated organization of signaling proteins. EMBO J. 21(23):6461-6472.