Technical Data
B0397-15A
BLNK, ID (B-cell Linker Protein, Cytoplasmic Adapter Protein, B-cell Adapter Containing a SH2 Domain Protein, B-cell Adapter Containing a Src Homology 2 Domain Protein, Src Homology 2 Domain-containing Leukocyte Protein of 65kD, SLP-65, SLP65, BASH, BLNK)
Description:
BLNK is a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in its gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the Center region of human BLNK (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human BLNK.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Imamura,Y., J. Biol. Chem. 284(15), 9804-9813 (2009). 2. Kamino,H., Cancer Sci. 99(12), 2444-2454 (2008).