Technical Data
Bcl 2 (B Cell Leukemia 2)
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (ECL): 5ug/ml; Titration is recommended for optimal results in individual systems.
Immunohistochemistry (Parrafin): 2.5ug/ml; Pressure cooker antigen retrieval is recommended. Use DAB detection
Immunohistochemistry (Frozen): 5ug/ml; No pre-treatment needed. Use DAB detection.
Optimal dilutions to be determined by the researcher.

Western Blot Controls:
Positive: MCF-7 cells
Negative: Normal rabbit IgG

IHC Controls:
Positive: Intestine or human lymph node
Negative: Brain tissue (only random cells will stain for Bcl-2)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic peptide corresponding to aa20-34 of human Bcl 2.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human Bcl 2. Particularly useful for the determination of Bcl 2 expression levels in species other than human. Species Crossreactivity: Mouse and opossum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.