Technical Data
B0807-06C
Bcl-2 (B Cell Leukemia 2)
Description:
Apoptosis regulator Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. At least 2 isoforms (alpha and beta) are produced by alternate splicing with different C-terminal ends.

Applications:
Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (formalin, paraffin, and frozen): 0.4-1.0ug/ml
Antigen retrieval may improve the staining.
Optimal dilution determined by the researcher.

Positive control:
Follicular and diffuse large B cell lymphoma tumors

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G317Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Synthetic peptide (GAAPAPGIFSSQPG) corresponding to aa41-54 from human Bcl-2 conjugated to thyroglobin.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Specificity:
Recognizes human Bcl-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.