Technical Data
Bcl-2 (B Cell Leukemia 2) (Biotin)
The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells.

Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 Nalm-6 tumor cells
Immunohistochemistry: Fixed, paraffin sections
Optimal dilutions to be determined by the researcher.

Flow Cytometry Protocol:
1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times.
2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells.
3. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06F at 5ug/ml.
4. Cells were then washed twice and incubated with S7973-88P Streptavidin (PE).
5. Cells were washed three times, fixed and analyzed by FACS.
6. Cells stained with a mean shift of 0.44 log10 fluorescent units when compared to a Mouse IgG1 negative control (Biotin) at a similar concentration.
7. This shift was blocked when permeabalized cells were preincubated 10 minutes with 20ul of 0.5mg/ml B0807-06E.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG17H161Affinity Purified
100ug-20CBlue IceHumanMouse
Synthetic peptide corresponding to aa41-54 of human Bcl-2.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Recognizes the intracellular human Bcl-2 protein at ~26kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Holz, L.E. et al., (2012) Hepatology DOI: 10.1002/hep.25821. 1. F. Pezzella, et al, (1990) Am J Pathol 137: 225-232. 2. I.A. Doussis, et al, (1993) Am J Clin Pathol 99: 663-667. 3. S. Cory, (1995) Annu Rev Immunol 13: 513-543.