Technical Data
B0807-06F
Bcl-2 (B Cell Leukemia 2) (Biotin)
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Technical Data |
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B0807-06F |
Bcl-2 (B Cell Leukemia 2) (Biotin) |
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Description: The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 Nalm-6 tumor cells Immunohistochemistry: Fixed, paraffin sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. 2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. 3. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06F at 5ug/ml. 4. Cells were then washed twice and incubated with S7973-88P Streptavidin (PE). 5. Cells were washed three times, fixed and analyzed by FACS. 6. Cells stained with a mean shift of 0.44 log10 fluorescent units when compared to a Mouse IgG1 negative control (Biotin) at a similar concentration. 7. This shift was blocked when permeabolized cells were preincubated 10 minutes with 20ul of 0.5mg/ml B0807-06E. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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