Technical Data
B0807-11F
Bcl 6, CT (B Cell Leukemia 6, Lymphoma 6, Zinc finger protein 51, LAZ3)
Description:
Bcl-6 (also known as LAZ3) is a member of the POZ/zinc finger (POK) family that contains an N-terminal POZ domain and 6 C-terminal zinc finger motifs (1). Bcl-6 is a transcriptional regulator that plays an important role in lymphocyte, myocyte, male germ cells, and keratinocyte survival and or differentiation (2-3). Bcl-6 has also been found to repress a number of genes involved in B cell activation and terminal differentiation, inflammation and cell cycle regulation (4). Chromosomal aberration in Bcl-6 may be a cause of B-cell non –Hodgkin lymphoma and B-cell leukemia (5).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-2000
Immunohistochemistry:1:50-1:100
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k40Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in the C-terminus of human Bcl-6.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human Bcl-6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kerckaert, J.-P., Deweindt, C., Tilly, H., Quief, S., Lecocq, G., and Bastard, C. (1993) Nat. Genet. 5, 66-70 2. Dent, A. L., Shaffer, A. L., Yu, X., Allman, D., and Staudt, L. M. (1997) Science 276, 589-592 3. Kumagai, T., Miki, T., Kikuchi, M., Fukuda, T., Miyasaka, N., Kamiyama, R., and Hirosawa, S. (1999) Oncogene 18, 467-475 4. Shaeffer, A. L., Yu, X., He, Y., Boldrick, J., Chan, E. P., and Staudt, L. M. (2000) Immunity 13, 199-212 5. Baron, B. W.; Nucifora, G.; McCabe, N.; Espinosa, R., III; Le Beau, M. M.; McKeithan, T. W. : Proc. Nat. Acad. Sci. 90: 5262-5266, 1993.