Technical Data
Bcl G (Bcl-2-like 14)
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain (reviewed in 1 and 2). BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G (3). The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein (3).

Can be used for detection of Bcl-G by Western blot at 2.5 to 5ug/ml.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
100ug-20CBlue IceHumanRabbit
Peptide corresponding to 15 amino acids near the C-terminus of human Bcl-G (GenBank accession no. NM_030766)
Purified by Ion Exchange chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human Bcl-G. Although this antibody should react with both isoforms, only the Bcl-GS protein has been observed. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cory S, et al. Oncogene 2003; 22:8590-607. 2. Heiser D, et al Exp. Geron.. 2004; 39:1125-35. 3. Guo B, et al. J. Biol. Chem. 2000; 276:2780-5.