Technical Data
B0899-03
BD1 (beta Defensin-1)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfide and a largely b-sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml, use control peptide to coat plates at 1ug/ml
Western Blot: 1-10ug/ml (ECL.)
Optimal dilutions to be determined by the researcher.

Control Peptide:
B0900-09A: BD1, Control Peptide (beta Defensin-1)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to 16aa near the N-terminus of mature mouse MBD-1, conjugated to KLH. Species Sequence Homology: rat, 87%. No significant homology exists with human beta-defensin 1 or other defensins.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA
Specificity:
Recognizes mouse BD-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Huttner, Km. et al., FEBS Lett. 413: 45-69 (1997). 2. Liu, L., et al., Genomics 43: 316-320 (1997). 3. Bartels, J., et al., Nature 387: 861 (1997). 4. Ganz, T., Science 286: 420 (1999). 5. Yang, D., et al., Science 286: 525 (1999).