Technical Data
B0901-01A
BD2 (Beta-defensin 2, HBD-2, BD-2)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.

The genes encoding human and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six beta-defensin cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. a-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ul/ml
Western Blot: 1-3ul/ml (ECL) using native or denatured protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGoat
Concentration:
As reported
Immunogen:
Recombinant corresponding to full length human Beta-Defensin 2 (Accession # O15263).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA.
Specificity:
Recognizes human BD2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harder J et al (1997) Nature 387, 861. 2. Diamond J. et al (2000) Infect. Immun. 68, 113- 119. 3. Morrision GM et al (1999) FEBS lett. 442, 112-116. 4. liu L et al (1998) Gene 222, 237-244. 5. Bartels J et al (1997) Nature 387, 861. 6. Ganz T (1999) Science 286, 420. 7. Yang D et al (1999) Science 286, 525.