Technical Data
B0903-26C
BD4, Recombinant, Human (Defensin, beta 104, HBD-4, BD-4, Beta-defensin 104, Beta-defensin 4, DEFB-4, DEFB104A, DEFB104, DEFB4, DEFB104B)
20ug
Molecular Biology Storage: -20CShipping: Blue Ice
Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. Four human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence (for BD-1 a propeptide region). Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta Defensin-4 Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 50aa. The BD-4 is purified by proprietary chromatographic techniques.

Recombinant protein corresponding to human BD-4, expressed in E. coli.

AA Sequence:
EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Molecular Weight:
~6kD

Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 0.1-50ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~6kD
Source: E. coli
Purity: ~98% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder in 20mM PBS, pH 7.4, 130mM sodium chloride. Reconstitute with sterile dH2O to 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.