Technical Data
B1082-13
BHMT (Betaine-homocysteine methyltransferase, BHMT)
Description:
e encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:16,000
Western Blot: 0.03-0.1ug/ml ~45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293.
Immunohistochemistry: 2-4ug/ml In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells.
Other dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See B1082-13-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-EQQLKELFEKQK, from the C Terminus of human BHMT (NP_001704.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human BHMT. Species Crossreactivity: mouse, rat. Species sequence homology: canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice., Collinsova M, Strakova J, Jiracek J, Garrow TA., J Nutr. 2006 Jun;136(6):1493-7.