Technical Data
Bile Esculin Azide Agar (Enterococcosel Agar) (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components listed as g/Liter:
Pancreatic Digest of Casein17.0
Peptic Digest of Animal Tissue3.0
Yeast Extract5.0
Oxgall, dehydrated10.0
Sodium Chloride5.0
Sodium Citrate1.0
Ferric Ammonium (HI) Citrate0.5
Sodium Azide0.25
Sodium azide inhibits Gram negative and Gram positive bacteria, other than enterococci, are inhibited by the bile salts.

Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Bile Esculin Azide Agar is a more selective medium as described by lsenberg. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Sodium azide inhibits Gram negative and Gram positive bacteria other than enterococci are inhibited by the bile salts.

Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original beige color.

Directions: 1. Dissolve 56grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121C (15 psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli (ATCC 25922)

Appearance: Light beige, homogeneous and free flowing

Solubility: Dark amber and turbid to clear.

pH: 7.1 0.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.