Technical Data
Bile Salts Mixture #3 (Powder)
Microbiological Grade
Storage RT    Shipping RT

Bile Salts Mixture #3 is a mixture of sodium cholate and deoxycholate derived from liver. Itís primary use is as a selective agent for the isolation of gram-negative microorganisms, inhibiting gram-positive cocci. It is soluble in distilled or dionized water and neutral to slightly alkaline.

Prepared Appearance (2% wt/vol): Clear, colorless to light amber, precipitate-free solution. pH (2% solution at 25deg.C)

Expected Cultural Response:

E. coli (ATCC 25922): Good to excellent growth, pink to red colonies surrounded by a zone of precipitated bile salts.

Staphylococcus aureus (ATCC 25923): Complete inhibition.

Test procedure: Refer to references below.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eaton, A.D., L.S. Clesceri and A.E. Greensberg (eds.), 1995. Standard Methods for the examination of water and wastewater, 19th ed. American Public Health Aoosication, Washington, D.C. 2. US Food and Drug Administration, 1995. Bacteriological Analytical Manual, 8th ed. AOAC International, Gaithersburd, MD. 3. Vanderzant, C. and D.F. Splittsosser (eds.) 1992. Compendium of Methods for the microbiological examination of food. American Public Health Association, Washington, D.C.