Technical Data
B1900
Blk
Description:
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of Blk (3). It has been suggested that Blk is involved in the control of B cell differentiation and proliferation (4,5). Except expressed in B cell, Blk transcripts were also detected in human thymocytes but not in mature T cells, implicating that Blk may play an important role in thymopoiesis (6).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding the C-terminus of human Blk (KLH).
Purity:
Purified by Protein A and immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes total levels of endogenous human Blk.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dymecki, S.M., et al., Science 247: 332-336 (1990). 2. Wasserman, R., et al., J. Immunol. 155: 644-651 (1995). 3. Sefton, B.M., et al., Curr. Opin. Immunol. 6: 372-379 (1994). 4. Malek, S.N., et al., Proc. Natl. Acad. Sci. USA 95: 7351-7356 (1998). 5. Yao, X.R., et al., Proc. Natl. Acad. Sci. USA 90: 7946-7950 (1993). 6. Islam, K.B., et al., J. Immunol. 154: 1265-1272 (1995).