		Technical Data
B1900	Blk

Description:
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of Blk (3). It has been suggested that Blk is involved in the control of B cell differentiation and proliferation (4,5). Except expressed in B cell, Blk transcripts were also detected in human thymocytes but not in mature T cells, implicating that Blk may play an important role in thymopoiesis (6).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to residues surrounding the C-terminus of human Blk (KLH).

Purity:

Purified by Protein A and immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Specificity:

Recognizes total levels of endogenous human Blk.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dymecki, S.M., et al., Science 247: 332-336 (1990). 2. Wasserman, R., et al., J. Immunol. 155: 644-651 (1995). 3. Sefton, B.M., et al., Curr. Opin. Immunol. 6: 372-379 (1994). 4. Malek, S.N., et al., Proc. Natl. Acad. Sci. USA 95: 7351-7356 (1998). 5. Yao, X.R., et al., Proc. Natl. Acad. Sci. USA 90: 7946-7950 (1993). 6. Islam, K.B., et al., J. Immunol. 154: 1265-1272 (1995).