Technical Data
B2106-70
Blimp-1 (BLIMP 1, BLIMP1, B Lymphocyte Induced Maturation Protein 1, Positive Regulatory Domain I Binding Factor 1, PR Domain Containing 1, PRDI BF1, PRDM1)
Description:
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence/Immunocytochemistry: 1:50-1:200
Flow Cytometry: 8ug/ml where cells are used at 2x10e6/ml
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
P3X (murine plasmocytoma) cell lysates

Cellular Localization:
Nuclear

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J333Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseMouse
Concentration:
~1mg/ml
Immunogen:
A fragment of the mouse Blimp-1 (residues 199-409).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 150mM sodium chloride, 0.1% sodium azide.
Specificity:
Recognizes mouse Blimp-1 at ~98kD and ~80kD (beta form). Species Crossreactivity: human and porcine (see Specific Reference).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lin, Y. et al. Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation. Science. 276: 596-599 (1997)
2. Lin, K. et al. Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells. Mol. & Cell. Biol. 22(13): 4771-4780 (2002)
3. Angelin-Duclos, C. et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo. J. Immunol. 165(10): 5462-5471(2000)
4. Ohinata, Y., et al. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature. 436: 207-213 (2005) (Mouse)