Technical Data
Media
Blood Agar Base, Low pH (Powder)
B2110-02
Microbiological Grade
Storage RT/4C    Shipping RT

Components shown as g/liter
Peptic Digest of Animal Tissue10.0
Sodium Chloride5.0
Heart Infusion Solids10.0
Agar15.0
Total:40.0g/liter
Cultivate a wide variety of bacteria, usually prepared with blood to observe hemolytic reactions.

Description:
These Blood Base media contain highly nutritious extracts or digests of beef. The beef products supply vitamins, carbohydrates, salts and other organic nitrogen compounds. Other peptones provide additional growth factors. Sodium chloride provides essential ions and electrolyte balance. Lowering pH to 6.8 enhances hemolytic reactions of streptococci.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 40grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121C (15 psi). 3. Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium.4. Cool to 45-50C. Dispense into Petri dishes or tubesStorage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli (ATCC 25922)

Appearance: Light beige, homogeneous and free flowing

Solubility: Cherry-red and opaque..

pH: 6.8 0.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.