		Technical Data
B2553-03J	Bone Morphogenetic Protein 1, NT (BMP 1, BMP1, BMP-1, FLJ44432, ProCollagen C-endopeptidase, Procollagen C-proteinase, PCP, pCP-2, PCOLC, Mammalian Tolloid, mTld, TLD)

Description:
In mammals the BMP-1 gene encodes both the BMP-1 protein and the mTld protein on two distinct, alternately spliced mRNAs. Both of these proteins exhibit procollagen C-proteinase activity and play a vital role in bone development and collagen formation. BMP-1 also cleaves C- and N- propeptides from other precursor proteins including procollagens V (2) and VII (1), chordin (3), prolysyl oxidase and probiglycan.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Human BMP-1 N-terminal region.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid, 0.05% sodium azide, 50% glycerol.

Specificity:

Recognizes human BMP-1 at ~65kD. May also detect some proteolytic fragments in some lysates.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Rattenholl, A. et al. (2002) Proteinases of the bone morphogenetic protein-1 family convert Procollagen VII to mature anchoring fibril collagen. J. Biol. Chem. 277(29): 26372-26378. 2. Imamura, Y. et al. (1998) Bone morphogenetic protein-1 processes the NH2-terminal propeptide, and a furin-like proprotein convertase processes the COOH-terminal propeptide of pro-alpha1(V) collagen. J Biol Chem 273(42): 27511-27517. 3. Scott, I.C. et al. (1999) Mammalian BMP-1/Tolloid- related metalloproteinases, including novel family member mammalian tolloid-like 2, have differential enzymatic activities and distributions of expression relevant to patterning and skeletogenesis. Dev. Biol. 213(2): 283-300.