Technical Data
B2553-03L
Bmp1, CT (Bone Morphogenetic Protein 1, BMP-1, Procollagen C-proteinase, PCP, Mammalian Tolloid Protein, mTld, PCOLC)
Description:
The BMP1 locus encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, BMP1 encodes a protein that is not closely related to other known growth factors. BMP1 protein and procollagen C proteinase (PCP), a secreted metalloprotease requiring calcium and needed for cartilage and bone formation, are identical. PCP or BMP1 protein cleaves the C-terminal propeptides of procollagen I, II, and III and its activity is increased by the procollagen C-endopeptidase enhancer protein. The BMP1 gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human Bmp1 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Bmp1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Leighton, M., et al., J. Biol. Chem. 278(20):18478-18484 (2003). 2.Hartigan, N., et al., J. Biol. Chem. 278(20):18045-18049 (2003). 3.Garrigue-Antar, L., et al., J. Biol. Chem. 277(45):43327-43334 (2002). 4.Rattenholl, A., et al., J. Biol. Chem. 277(29):26372-26378 (2002). 5.Janitz, M., et al., J. Mol. Med. 76(2):141-146 (1998).