		Technical Data
B2553-06A	Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant	2ug 10ug

Growth Factors, Cytokines

Storage: -20°CShipping: RT

Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

Recombinant human Bone Morphogenetic Protein-2 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.

Biological Activity:
The ED50 as determined by the cytolysis of MC3T3-E1 cells is <50ng/ml corresponding to a specific activity of 2x10e4 IU/mg.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution.
2. Analysis by RP-HPLC, using a standard solution of BMP2 as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 100mM acetic acid. Add 0.1% HSA or BSA for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
26018D

Source: E. coli
Purity: 95% by RP-HPLC and SDS-PAGE Silver Stain. Chromatographically purified.
Form: Supplied as a powder lyophilized from 10mM sodium citrate, pH 3.5. Reconstitute with sterile 100mM acetic acid to 0.1mg/ml. Add 0.1% HSA or BSA for long term storage. This solution can then be diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Reppe, S., et al., Biochem. Biophys. Res. Commun. 324(1): 218-223 (2004). 2. Kim, Y.J., et al., J. Biol. Chem. 279(49): 50,773-50,780 (2004). 3. Seeherman, H.J., et al., J. Bone Joint Surg. Am. 86-A(9): 1961-1972 (2004). 4. Kaden, J.J., et al., J. Heart Valve Dis. 13(4): 560-566 (2004). 5. Mummaneni, P.V., et al., J. Neurosurg. Spine 1(1): 19-23 (2004). 6. Wajih, N., et al., J. Biol. Chem. 279(41): 43,052-43,060 (2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.