Technical Data
B2553-08B
Bone Morphogenetic Protein 2, Recombinant, Human (BMP-2) (Human 293 Cells)
10ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
BMP-2 is expressed in human 293 cells as a glycosylated dimer with an apparent molecular mass of 28-35kD.

Recombinant protein corresponding to Human BMP-2 expressed in human 293 cells.

Activity:
The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (Mouse chondrogenic cell line).

Specific Activity:
~20-100ng/ml ED50

Endotoxin: 1EU/ug (LAL)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 4mM HC, 0.1% endotoxin-free recombinant HSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
30-38kD
Source: Recombinant Human BMP-2 expressed in human 293 cells.
Purity: 95% as determined by SDS-PAGE and stained with Coomassie blue

Form: Supplied as a lyophilized powder from PBS. Reconstitute with sterile 4mM HCl, 0.1% endotoxin-free recombinant HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Chen, D. et al. Growth Factors 22; 233-241 (2004) 2. Nakamura k etal. Exp cell Research 250; 351-363 (1999).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.