Technical Data
B2553-08W
Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant, Western Blot Positive Control
100ul
Molecular Biology Storage: -20CShipping: Blue Ice
The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and 13 (CDMP-2 / GDF-6) may be involved in later stages of skeletal formation.
BMP2 or BMP-2A, a 396aa protein in human (chr 20p12) belongs to the TGF-beta family, It is involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues. It is abundant in lung, spleen, and colon.

Source:
Recombinant human BMP-2 protein

BMP-2 Protein: The DNA sequence encoding human BMP-2 was expressed in CHO cells. The mature recombinant human BMP-2 generated by the proteolytic removal of the signal peptide subunits. The purified protein is of ~26kD, due to glycosylation the rhBMP-2 migrates as an ~36kD protein under non-reducing conditions and as 17-18kD protein under reducing conditions in SDS-PAGE.

Applications:
Western Blot: Load 10ul/lane of B2553-50C for good visibility with antibody Cat # B2553-50D.

Note: SDS may crystallize in cold conditions. It should redissolved by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2X sample buffer per 10ul of the B2553-50C solution prior to heating and loading on gels. This preparation is not biologically active. It is not suitable for ELISA or other applications where native protein is required. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Do not freeze, thaw, or heat repeatedly

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Purified
Concentration: Not determined
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.) Kawabata, M et al (1998) Cytokine and Growth Factor Reviews 9: 49-61,
2.) Ebendal, T et al (1998), J. Neurosci. Res. 51: 139-146;
3.) Reddi, A. H (1998), Nature Biotechnology 16: 247-252.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.