		Technical Data
B2570-22	Borrelia burgdorferi (Lyme Disease) (Biotin)

Description:
Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system
Immunohistochemistry: formalin fixed/paraffin embedded. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%. Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Rabbit

Concentration:

~4-5mg/ml

Immunogen:

Whole cell preparation from B. burgdorferi.

Purity:

Purified IgG fraction

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Covalently coupled with the N-Hydroxysuccinimide ester of Biotin.

Specificity:

Western Blot bands at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Crossreacts with Treponema pallidum, B. hermsii, B. parkerii.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.